The instrument takes advantage of modern ccd technology combined with the latest generation of readout electronics. Spectramax m3, m4, m5, and m5e multimode microplate. Molecular devices spectramax 250 microplate reader. Sop molecular devices spectramax 190 absorbance plate reader hplc agilent 1100.
Replacement parts and additional breastshield sizes are available to purchase on the spectra website. The cookie is used to remember the user consent for the cookies under the category analytics. The spectramax quickdrop micro volume spectrophotometer addresses the needs of life science researchers who quantify very small amounts of dna, rna, oligos, and proteins. The glomax 96 microplate luminometer software is easy to use. Optics mirrored optics focus the light into the sample volume, and cutoff filters are used to reduce stray light and minimize background interference. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. The adenylate kinase released from serial dilutions of k562 cells measured using the combination of the toxilight assay and the spectramax l luminometer. Spectramax plus 384 microplate reader manual conquer. Using the molecular devices test plate to calibrate the spectramax reader. Softmax, flexstation, flipr, softmax, spectramax, analyst, and vmax are registered trademarks and. Level the laser using the bulls eye as a reference. As a standalone unit, the spectramax 250 can obtain readings in either endpoint or kinetic modes. With optimized reagents and the industryleading data acquisition and analysis tool, softmax pro 7 software, the spectramax id3 allows users to customize workflow to perfectly. Please refer to the instrument manuals for additional instructions related to the usbonly instruments from molecular devices.
The 4 injector configuration enables flash and glow bret assays and a 2fold improvement in speed for traditional flash and glow assays. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Spectramax and versamax plate readers operators manual 01120126 rev a. These settings are also valid for the spectramax m3m4, flexstation 3 multimode. When leveling the ll200, be sure not to rotate the laser distance to a wall is 6 m 20 ft. Spectramax microplate spectrophotometer operators manual. Spectramax l microplate reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, g proteincoupled receptor gpcr in 96 and 384well plates. The spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The spectramax l reader is available in 1 and 2channel photomultiplier tube configurations. The manual pmt calibration method is based on readings of 4 wells from the assay itself. For example, to prevent precipitation from clogging injector lines, the lines.
Compact designs make these instruments very compatible with small benchtop workspaces. In this user guide, all references to spectramax multimode. The cuvette chamber spectramax plus 384 only figure 1. The automatic pmt calibration method is based on readings of 4 internal leds at 395, 470, 527, and 570 nm. In this manual, we use the terms absorbance and optical density interchangeably. Operators manual molecular devices spectramax 190 absorbance plate reader manual. Precise and reliable autowashing injectors the spectramax l readers nextgeneration injectors enable better results and more robust performance over current offerings. The spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. When a feature or capability applies to only certain readers, this exception is noted. Spectramax quickdrop microvolume spectrophotometer. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. Spectramax m3 microplate reader from molecular devices. Rapid, accurate dna, rna, and protein quantitation in a one touch, full spectrum micro volume absorbance reader.
The software comes preloaded with protocols for promega luminescent assays. Located at the right front of the spectramax plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. In this user guide, all references to spectramax multimode microplate readers include the m3, m4, m5, and m5e models. Never perform any operation on the instrument in an. Setup guide on the molecular devices spectramax m5m5e. The stateoftheart spectramax l microplateluminometer helps in measurement of flash and glow assays, including dual luciferase reporter gene, g proteincoupled receptor gpcrviaaequorin,bioluminescence resonance energy transfer bret and acridinium ester flash assays, in both 96 and 384well plates. The spectramax l reader can give up to a sixfold increase in.
Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax plus384 microplate spectrophotometer operators manual. Multichannel precision pmt to pmt rlu precision on four wells ranging in brightness from 8,000 to 1,600,000 rlu read on a 6channel spectramax l reader using the automatic internal led pmt calibration method. Introduction softmax pro software controls molecular devices spectrophotometers, absorbance, luminescence, and fluorescence microplate re adersproviding extensive data calculation and analysis capabilities under a glpgmp work environment for pharmaceutical, bio. This cookie is set by gdpr cookie consent wordpress plugin. Spectramax l microplate reader molecular devices berner. The spectramax l microplate luminometer from molecular devices. The 2channel configuration is available with 0, 2, or 4 injectors. Spectramax and versamax plate readers operators manual 01120126 rev a 5 1. Spectramax id3 multimode microplate reader, molecular. Spectramax 190 microplate spectrophotometer operators manual iii labor and parts to obtain warranty service during the applicable warranty period, you must take the product or deliver the product properly packaged in the original shipping materials and carton to an authorized molecular devices corporation service facility. Automatic and manual pmt calibration the spectramax l reader offers two methods to correct inherent pmt spectral response variability in multichannel configurations. Molecular devices spectramax plus384 microplate reader manual attachments molecular devices spectramax plus384 microplate reader manual 624 kb. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system.
Using the molecular devices test plate to calibrate the. Up to nine userdefined assay protocols can be saved in nonvolatile memory for future use with settings including read mode, wavelengths, auto mix state, blank pattern, and temperature setpoint. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Software for the test plate must be installed on the control computer before the plate can be used. Find the user manual you need for your tools and more at manualsonline. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. The spectramax l microplate reader measures flash and glow luminescence assays in both 96 and 384well plates. The following document is intended to demonstrate setup of this instrument. The spectramax l reader offers two methods to correct inherent pmt spectral response. Spectraplus users guide spectraplus fft spectral analysis system version 5. Molecular devices exdemo spectramax l with 2 injectors. Spectramax l mikroplattenreader, luminometer, lumineszenz.
The spectromaxx enables the accurate analysis of copper and its alloys. Spectramax m2m2e user guide university of michigan. It is also possible to create, store and retrieve userdefined protocols. Spectramax plus 384 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax plus 384 control panel are shown in boxed helvetica type. Molecular devices exdemo spectramax l with 2 injectors sensitive luminometer with programmable injector options for 96 and 384well microplate. Up to nine user defined assay protocols can be saved in nonvolatile memory for future use with settings including read mode, wavelengths, auto mix state, blank pattern, and temperature setpoint. Spectramax m3, m4, m5, and m5e multimode microplate reader. With optimized reagents and the industryleading data acquisition and analysis tool, softmax pro 7 software, the spectramax to perfectly match your needs. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Spectramax l microplate reader, luminometer, luminescence.
Softmax pro software user guide biological discovery in. In no event will mdc be liable to the user for any damages, including any lost profits, lost savings, or other. Molecular devices spectramax m2 user guide and manual. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type. Spectramax l microplate reader from molecular devices, llc. Spectramax l luminescence microplate reader from molecular devices llc. It is upgradeable to include a barcode reader, programmable injectors, and dualchannel configurations for a 2fold increase for medium throughput.
The innovative optical system covers the entire usable wavelength range to enable selection of the best analytical wavelengths paired with reference lines for. The spectramax l reader can give up to a sixfold increase in throughput with multi. System includes spectramax l luminometer for reading 96 and 384 well microplates with current version of softmax pro software, autorange for 9 orders of dynamic range, photon counting for high sensitivity, landscapeportrait plate reading, incubator, user manual, usb interface cable, 1 year mdcare phd technical support plan and 1 year warranty covering parts and labour. This directtoexcelbased software gives the user flexibility in data retrieval, storage, and analysis.
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